A DP-like transcription factor protein interacts with E2fl1 to regulate meiosis in Tetrahymena thermophila. Issue 5 (4th March 2018)

• Record Type: Journal Article
• Authors: Zhang, Jing; Yan, Guanxiong; Tian, Miao; Ma, Yang; Xiong, Jie; Miao, Wei
• Is Part Of: Cell cycle. Volume 17:Issue 5(2018)
• Page Start: 634
• Page End: 642
• Publication Date: 2018-03-04
• Subjects: Tetrahymena thermophila -- meiosis -- transcription factor -- E2fl1/Dpl2 complex -- network; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2018.1431595
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Evolutionarily conserved E2F family transcription factors regulate the cell cycle via controlling gene expression in a wide range of eukaryotes. We previously demonstrated that the meiosis-specific transcription factor E2fl1 had an important role in meiosis in the model ciliate Tetrahymena thermophila . Here, we report that expression of another E2F family transcription factor gene DPL2 correlates highly with that of E2FL1 . Similar to e2fl1 Δ cells, dpl2 Δ cells undergo meiotic arrest prior to anaphase I, with the five chromosomes adopting an abnormal tandem arrangement. Immunofluorescence staining and immunoprecipitation experiments demonstrate that Dpl2 and E2fl1 form a complex during meiosis. We previously identified several meiotic regulatory proteins in T. thermophila . Cyc2 and Tcdk3 may cooperate to initiate meiosis and Cyc17 is essential for initiating meiotic anaphase. We investigate the relationship of these regulators with Dpl2 and E2fl1, and then construct a meiotic regulatory network by measuring changes in meiotic genes expression in knockout cells. We conclude that the E2fl1/Dpl2 complex plays a central role in meiosis in T. thermophila.