Development of a large‐volume human‐derived adipose acellular allogenic flap by perfusion decellularization. Issue 2 (19th May 2018)

• Record Type: Journal Article
• Authors: Giatsidis, Giorgio; Guyette, Jacques P.; Ott, Harald C.; Orgill, Dennis P.
• Is Part Of: Wound repair and regeneration. Volume 26:Issue 2(2018)
• Page Start: 245
• Page End: 250
• Publication Date: 2018-05-19
• Subjects: Wound healing -- Periodicals; Regeneration (Biology) -- Periodicals; 617.14
• DOI: 10.1111/wrr.12631
• Languages: English
• ISSNs: 1067-1927

Abstract

Abstract: In reconstructive surgery, transfer of patients' tissue (autologous flaps) is routinely used to repair large soft tissue defects caused by surgery, trauma, chronic diseases, or malformations; unfortunately, this strategy is not always possible and often creates a secondary defect in the donor site of the tissue. Tissue‐engineered synthetic flaps are currently unable to repair clinically‐relevant, large‐volume defects; allogenic flaps from cadaveric donors could provide a ready‐to‐use biological alternative if treated with methods to avoid the immune‐rejection of the donor's cells. Here, we describe the successful decellularization of a large (> 800 cc) human‐derived adipose flap through a perfusion apparatus; we demonstrate the complete removal of the immunogenic cellular components of the flap with the retention of its structural components and vascular network. Our aim is to obtain a universally compatible, off‐the‐shelf acellular allogenic flap that could be recellularized with cells from recipient patients to provide a tissue‐engineered allogenic/autologous alternative for reconstruction of large‐volume soft‐tissue defects.