Abiotic Sequence‐Coded Oligomers as Efficient In Vivo Taggants for the Identification of Implanted Materials. Issue 33 (5th July 2018)

• Record Type: Journal Article
• Authors: Karamessini, Denise; Simon‐Yarza, Teresa; Poyer, Salomé; Konishcheva, Evgeniia; Charles, Laurence; Letourneur, Didier; Lutz, Jean‐François
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 33(2018)
• Page Start: 10574
• Page End: 10578
• Publication Date: 2018-07-05
• Subjects: anti-counterfeiting technologies -- biomedical implants -- information-containing macromolecules -- sequence-defined polymers -- sequencing; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201804895
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Sequence‐defined oligourethanes were tested as in vivo taggants for implant identification. The oligomers were prepared in an orthogonal solid‐phase iterative approach and thus contained a coded monomer sequence that can be unequivocally identified by tandem mass spectrometry (MS/MS). The oligomers were then included in small amounts (1 wt %) in square‐centimeter‐sized crosslinked poly(vinyl alcohol) (PVA) model films, which were intramuscularly and subcutaneously implanted in the abdomen of rats. After one week, one month, or three months of implantation, the PVA films were explanted. The rat tissues exposed to the implants did not exhibit any adverse reactions, which suggested that the taggants are not harmful and probably not leaching out from the films. Furthermore, the explanted films were immersed in methanol, as a solvent for oligourethanes, and the liquid extract was analyzed by mass spectrometry. In all cases, the oligourethane taggant was detected, and its sequence was identified by MS/MS. Abstract : Uniform oligourethanes with defined information sequences were included in crosslinked poly(vinyl alcohol) model films that were implanted in live rats. After three months, the tagged films were explanted, and the coded sequence of the oligourethane taggant was unambiguously identified by tandem mass spectrometry.