Label‐free non‐linear microscopy to measure myelin outcome in a rodent model of Charcot‐Marie‐Tooth diseases. Issue 12 (5th September 2018)

• Record Type: Journal Article
• Authors: Hajjar, Helene; Boukhaddaoui, Hassan; Rizgui, Amel; Sar, Chamroeun; Berthelot, Jade; Perrin‐Tricaud, Claire; Rigneault, Herve; Tricaud, Nicolas
• Is Part Of: Journal of biophotonics. Volume 11:Issue 12(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-09-05
• Subjects: demyelinating diseases -- myelin sheath -- non‐linear optical microscopy -- peripheral nervous system -- Schwann cells; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201800186
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : Myelin sheath produced by Schwann cells covers and nurtures axons to speed up nerve conduction in peripheral nerves. Demyelinating peripheral neuropathies result from the loss of this myelin sheath and so far, no treatment exists to prevent Schwann cell demyelination. One major hurdle to design a therapy for demyelination is the lack of reliable measures to evaluate the outcome of the treatment on peripheral myelin in patients but also in living animal models. Non‐linear microscopy techniques which include second harmonic generation (SHG), third harmonic generation (THG) and coherent anti‐stokes Raman scattering (CARS) were used to image myelin ex vivo and in vivo in the sciatic nerve of healthy and demyelinating mice and rats. SHG did not label myelin and THG required too much light power to be compatible with live imaging. CARS is the most reliable of these techniques for in vivo imaging and it allows for the analysis and quantification of myelin defects in a rat model of CMT1A disease. This microscopic technique therefore constitutes a promising, reliable and robust readout tool in the development of new treatments for demyelinating peripheral neuropathies. Abstract : Measuring the treatment outcome of chronic diseases such as the hereditary peripheral neuropathies is not straightforward. It requires specific analyses among which imaging of peripheral nerve myelin. In this paper myelin of healthy, injured and diseased small rodents was imaged ex vivo and in vivo using several non‐linear microscopy approaches. Coherent anti‐stokes Raman scattering microscopy allows for the qualitative and quantitative assessment of peripheral nerve myelin defects in living diseased animals.