Tailormade Polysaccharides with Defined Branching Patterns: Enzymatic Polymerization of Arabinoxylan Oligosaccharides. Issue 37 (19th August 2018)

• Record Type: Journal Article
• Authors: Senf, Deborah; Ruprecht, Colin; Kishani, Saina; Matic, Aleksandar; Toriz, Guillermo; Gatenholm, Paul; Wågberg, Lars; Pfrengle, Fabian
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 37(2018)
• Page Start: 11987
• Page End: 11992
• Publication Date: 2018-08-19
• Subjects: carbohydrates -- enzymes -- glycosynthases -- structure elucidation -- synthetic methods; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201806871
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The heterogeneous nature of non‐cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase‐catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X‐ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure–property relationship studies that are not accessible by other means. Abstract : Designer polysaccharides : Enzymatic polymerization of synthetic arabinoxylan oligosaccharides provided artificial polysaccharides with systematically altered branching patterns. These well‐defined arabinoxylans constitute a unique set of tools for structure–property relationship studies regarding crystallinity and affinity to other cell wall components.