P403 Treatment naïve newly diagnosed patients with Crohn's disease have microbial dysbiosis correlated with disease activity and faecal calprotectin—results from a prospective inception cohort. (16th January 2018)

• Record Type: Journal Article
• Authors: Yanai, H; Goren, I; Reshef, L; Godny, L; Yadgar, K; Zonenesain, K; Dotan, I
• Is Part Of: Journal of Crohn's and colitis. Volume 12:Number 1(2018:Jan.)Supplement 1
• Page Start: S308
• Page End: S309
• Publication Date: 2018-01-16
• Subjects: Inflammatory bowel diseases -- Periodicals; 616.344005
• DOI: 10.1093/ecco-jcc/jjx180.530
• Languages: English
• ISSNs: 1873-9946

Abstract

Abstract: Background: Microbial dysbiosis is believed to play a role in Crohn's disease (CD). Most data are derived from CD patients under medications, an exposure that might impact microbial composition. Our aim was to assess microbial dysbiosis in patients with newly diagnosed CD and correlate it with disease activity. Methods: Newly diagnosed CD patients were prospectively recruited and followed longitudinally. Clinical data, disease activity (physician global assessment [PGA] ranging from 0 to 3), and serum and faecal inflammatory biomarkers, were collected. Faecal samples were assessed for microbial composition using 16S rRNA sequencing. The microbial dysbiosis index (MDI) was used to quantify the degree of dysbiosis per sample. Results: Overall 23 treatment naïve newly diagnosed CD patients had a complete set of data. Distinct separation in microbial composition between patients with low and high PGA was observed by the analysis of similarities test ( p = 0.016, R = 17%). Patients with low PGA had higher microbial diversity as well as lower MDI and faecal calprotectin levels compared with those with high PGA (median Shannon: 3.1 vs. 2.7, p = 0.04; median calprotectin: 293 vs. 595 micrograms/g, p = 0.04; median MDI: −1.6 vs. −0.5; p = 0.006). Samples from patients with high PGA were richer in Enterobacteriaceae, while samples from patients with low PGA scores were richer in Bacteroidaceae, Ruminococcaceae, and Lachnospiraceae. Abundance of Bacteroidales order negatively correlated with calprotectin (FDR-adjusted p : 0.04, R = −0.5). Conclusions: Microbial dysbiosis in treatment naïve newly diagnosed CD patients was associated with disease activity as reflected by PGA and faecal calprotectin. Longitudinal assessment may reveal specific early dysbiosis patterns as predictive biomarkers for disease flares.