The complete chloroplast genome sequence of Cremastra appendiculata (Orchidaceae) revealed by next-generation sequencing and phylogenetic implication. Issue 2 (3rd July 2018)

• Record Type: Journal Article
• Authors: Mao, Shao li; Zhou, Yafu; Yuan, Hao; Lu, Chao; Chang, Huihui
• Is Part Of: Mitochondrial DNA. Volume 3:Issue 2(2018)
• Page Start: 1108
• Page End: 1109
• Publication Date: 2018-07-03
• Subjects: Cremastra appendiculata -- chloroplast genome -- endangered species; Mitochondrial DNA -- Periodicals; 572.869
• DOI: 10.1080/23802359.2018.1516122
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: The complete chloroplast genome sequence of Cremastra appendiculata, a rare and endangered species of China, was determined by Illumina pair-end sequencing. The results showed that the complete plastid genome was 160, 494 bp in length, containing a large single copy (LSC) of 88, 249 bp and a small single copy (SSC) of 21, 457 bp, which were separated by a pair of 25, 394 bp inverted repeats (IRs). A total of 132 unique genes were annotated, including 86 protein coding genes, 38 tRNA genes, and 8 rRNA genes. Among these genes, 18 genes contained one or two introns. The overall GC contents of the plastid genome were 36.9%, and in the IR regions, LSC and SSC were 43.8, 34.4 and 30.9%, respectively. Maximum likelihood analysis revealed that the new sequenced species C. appendiculata was closer to the species in Cattleya and formed one clade with a high bootstrap value at the middle of the phylogenetic tree of Orchidaceae.