Sonchus oleraceus Linn protects against LPS-induced sepsis and inhibits inflammatory responses in RAW264.7 cells. (23rd May 2019)

• Record Type: Journal Article
• Authors: Chen, Lei; Lin, Xiujun; Xiao, Jianbo; Tian, Yuting; Zheng, Baodong; Teng, Hui
• Is Part Of: Journal of ethnopharmacology. Volume 236(2019)
• Page Start: 63
• Page End: 69
• Publication Date: 2019-05-23
• Subjects: Sonchus oleraceus Linn -- Chlorogenic acid -- Caffeic acid -- Anti-inflammation MAPKs; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2019.02.039
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Sonchus oleraceus Linn (SOL) belongs to family of Asteraceae, is a traditional medicinal plant, which has been used to treat tumor, inflammatory diseases, infection and so on in Chinese folk culture. Aim of the study: This work investigated the influence of aqueous ethanol extract of whole plant of SOL and contribution of its main components on inflammation Methods and results: Oral administration of SOL (10 mg/kg) to mice reduced the expression of inflammatory cytokines including IL-6, IL-1β, and TNF-α, in the LPS-induced sepsis mouse model. Major phenolics in SOL were isolated and determined by HPLC. Results indicate that SOL at the concentration range from 25 to 100 μg/mL and its main components, chlorogenic acid, caffeic acid (25–100 μM) significantly reduced the pro-inflammatory cytokine IL-1β, IL-6, TNF-α, attenuated iNOS and COX-2 expression in LPS-stimulated Macrophages. In addition, western blot analysis showed SOL suppressed inducible nitric oxide synthase (iNOS) protein expression and the phosphorylation of extracellular signal-regulated kinase (ERK), p38, c-Jun N-terminal kinase (JNK). Conclusion: The underlying mechanism of anti-inflammation might be in according with the inhibition of MAPKs activation as well as down regulation of iNOS and cyclooxygenase-2 (COX-2). Graphical abstract: fx1