ITRAQ-based comparative proteome analysis for molecular mechanism of defense against acute ammonia toxicity in Pacific White shrimp Litopenaeus vannamei. Issue 74 (March 2018)

• Record Type: Journal Article
• Authors: Lu, Xia; Luan, Sheng; Dai, Ping; Meng, Xianhong; Cao, Baoxiang; Luo, Kun; Kong, Jie
• Is Part Of: Fish & shellfish immunology. Issue 74(2018)
• Page Start: 52
• Page End: 61
• Publication Date: 2018-03
• Subjects: Litopenaeus vannamei -- Ammonia toxicity tolerance -- Molecular mechanism -- Comparative proteome analysis -- iTRAQ; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.12.030
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: In the practical farming of Litopenaeus vannamei, the intensive culture system and environmental pollution usually results in a high concentration of ammonia, which brings large detrimental effects to shrimp, such as increasing the susceptibility to pathogens and even causing high mortality. We have revealed that the survival time under acute ammonia stress varied substantially among different families and obtained ammonia-tolerant (LV_T) and ammonia-sensitive (LV_S) families. In order to understand the molecular mechanism of defense against ammonia toxicity in shrimp, we performed iTRAQ LC-MS/MS proteomic analysis between LV_T and LV_S groups of L. vannamei under acute ammonia stress to identify the key proteins and pathways that play an effective role for against ammonia toxicity. By comparative proteome analysis, 202 significantly differentially proteins (DEPs) were identified in LV_T compared to LV_S, and most of the DEPs (60%) were up-regulated. Excepting for the proteins without function reporting, the meaningful finding is that 77.8% of the DEPs have been reported mainly involving in immune defense and stress tolerant in crustacean species, such as hemocyanin, Rab7, Rab GTPase, Rac1, alpha 2 macroglobulin, Bip, peroxiredoxin, Cu/Zn SOD, glutathione peroxidase, thioredoxin, calreticulin, and Elongation Factor 1-alpha, etc. These DEPs might potentially play important role in against ammonia toxicity, and it also reflected a relation between ammonia tolerance and pathogen resistance. In addition, a total of 10 significantly changed KEGG pathways were detected, and the network diagram of these KEGG pathways showed that more critical nodes were up-regulated, which involved in protein synthesis and transport, and against stress stimuli. This study provided important information for understanding the molecular mechanism of defense against ammonia toxicity in shrimp at whole protein level. Highlights: The mechanism of against ammonia toxicity in shrimp analyzed by iTRAQ approach. Ammonia tolerant and sensitive shrimp used for the comparative protein analysis. Most of the significantly different proteins involved in immune and up regulated. Most of the critical nodes in the significantly changed pathways were up regulated. A relation between ammonia tolerance and pathogen resistance was also suggested.