Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Issue 74 (March 2018)

• Record Type: Journal Article
• Authors: Gobi, Narayanan; Vaseeharan, Baskaralingam; Chen, Jiann-Chu; Rekha, Ravichandran; Vijayakumar, Sekar; Anjugam, Mahalingam; Iswarya, Arokiadhas
• Is Part Of: Fish & shellfish immunology. Issue 74(2018)
• Page Start: 501
• Page End: 508
• Publication Date: 2018-03
• Subjects: Probiotics -- Tilapia -- Innate immunity -- Serum -- Mucus -- Antioxidant -- Aeromonas hydrophila; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.12.066
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The present study evaluated the dietary supplementation of probiotic Bacillus licheniformis Dahb1 on the growth performance, immune parameters and antioxidant enzymes activities in serum and mucus as well as resistance against Aeromonas hydrophila in Mozambique tilapia Oreochromis mossambicus . Fish (24 ± 2.5 g) were fed separately with three diets, 1) commercial diet (control), 2) diet containing probiotic at 10 5 cfu g −1 (D1) and 3) diet containing probiotic at 10 7 cfu g −1 (D2) for 4 weeks. Growth performance in term of final weight (FW) specific growth rate (SGR) and feed conversion ratio (FCR), immune parameters of total protein (TP), alkaline phosphatase (ALP), myeloperoxidase (MPO), lysozyme (LYZ), reactive oxygen species (ROS), reactive nitrogen species (RNS) and antioxidant parameters of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in serum and mucus were evaluated after 2 nd and 4 th weeks. The FW, SGR, and FCR of fish fed with D1 and D2 significantly improved ( p < 0.05). The activities of ALP, LYZ and MPO in the mucus were significantly higher ( p < 0.05) in fish that fed D1 and D2. The TP, ROS, RNS, SOD and GPx in the serum were significantly higher ( p < 0.05) in fish that fed D1 and D2. In addition, the challenge test showed that fish fed D1 and D2 enhanced significantly ( p < 0.05) the resistance against A. hydrophila (1 × 10 7 cells ml −1 ). In conclusion, probiotic B. licheniformis Dahb1 can be applied in diet at 10 7 cfu g −1 to improve healthy status and resistance against A. hydrophila in tilapia farming. Graphical abstract: Image 1 Highlights: Bacillus licheniformis Dahb1 could be used as feed additive to O. mossambicus . The growth performance of fish fed with the diet containing B. licheniformis Dahb1 significantly improved. Antioxidant and immune parameters in mucus and serum enhanced in B. licheniformis Dahb1 groups. Dietary supplementation of probiotic improved survival rate of O. mossambicus against A. hydrophila challenge. The optimal dose of dietary supplementation of B. licheniformis Dahb1 was 10 7 cfu g −1 diet.