A comparative study on oxidative stress response in the hepatopancreas and midgut of the white shrimp Litopenaeus vannamei under gradual changes to low or high pH environment. Issue 76 (May 2018)

• Record Type: Journal Article
• Authors: Han, Si-yin; Wang, Meng-qiang; Wang, Bao-jie; Liu, Mei; Jiang, Ke-yong; Wang, Lei
• Is Part Of: Fish & shellfish immunology. Issue 76(2018)
• Page Start: 27
• Page End: 34
• Publication Date: 2018-05
• Subjects: Litopenaeus vannamei -- Hepatopancreas -- Midgut -- Gradual-low pH -- Gradual-high pH -- Antioxidant; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.02.001
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: White shrimp Litopenaeus vannamei were reared under conditions of gradual changes to a low pH (gradual-low pH, 6.65–8.20) or a high pH (gradual-high pH, 8.20–9.81) versus a normal pH environment (8.14–8.31) during a 28-day period. Survival of shrimp, and ROS production, antioxidant responses and oxidative damage in the hepatopancreas and midgut were investigated. Consequently, shrimp enhanced MnSOD, GPx, and Hsp70 transcripts as early defense mechanism in the hepatopancreas and midgut to scavenge excessive ROS during short-term (≤ 7 days) gradual-low and high pH stress. Meanwhile, the hepatopancreas was more sensitive to ROS than midgut because of earlier ROS production increase, antioxidant response and oxidative damage. Then, suppressed antioxidant response in the hepatopancreas and midgut of shrimp suggested a loss of antioxidant regulatory capacity caused by aggravated oxidative damage after long-term (≥ 14 days) gradual-high pH stress, leading to continuous death. However, enhanced GPx, GST, and Hsp70 transcripts in the hepatopancreas and midgut might be long-term(≥ 14 days) antioxidant adaptation mechanism of shrimp to gradual-low pH stress, which could prevent further ROS perturbation and weaken oxidative damage to achieve a new immune homeostasis, contributing to stable survival rate. Therefore, we have a few insights that it is necessary to protect hepatopancreas for controlling shrimp death under gradual-high pH stress. Highlights: The hepatopancreas in shrimp was more sensitive to ROS than midgut under pH stress. Shrimp could lose antioxidant regulatory capacity after long-term gradual-high pH stress. Enhanced antioxidant response might be long-term antioxidant adaptation mechanism of shrimp to gradual-low pH stress. It is necessary to protect hepatopancreas for controlling shrimp death under gradual-high pH stress.