Exploring on the bioactive markers of Codonopsis Radix by correlation analysis between chemical constituents and pharmacological effects. (23rd May 2019)

• Record Type: Journal Article
• Authors: Gao, Shiman; Liu, Jiushi; Wang, Min; Liu, Yunbao; Meng, Xiangbao; Zhang, Tao; Qi, Yaodong; Zhang, Bengang; Liu, Haitao; Sun, Xiaobo; Xiao, Peigen
• Is Part Of: Journal of ethnopharmacology. Volume 236(2019)
• Page Start: 31
• Page End: 41
• Publication Date: 2019-05-23
• Subjects: CR Codonopsis Radix -- TCM traditional Chinese medicine -- UPLC-ESI-Q-TOF-MS/MS ultra performance liquid chromatography-electrospray ion source-quadrupole-time of flight-tandem mass spectrometry -- PCA principal component analysis -- CCA canonical correlation analysis -- IMPLAD institute of medicinal plant development -- Cy cyclophosphamide -- rhG-CSF recombinant human granulocyte colony-stimulating factor -- ELISA enzyme-linked immuno sorbent assay -- RCLB red blood cell lysis buffer -- CNKI Chinese national knowledge infrastructure -- UV ultra violet -- i.p. intraperitoneal injection -- i.g. intragastric injection -- i.h. hypodermic injection -- WBC white blood cell count -- RBC red blood cell count -- HGB hemoglobin content -- PLT platelet count -- RET reticulocyte count -- BMNC bone marrow nucleated cell; Codonopsis Radix -- UPLC-ESI-Q-TOF-MS/MS -- UPLC fingerprint -- Hematopoietic function -- Immunologic function -- Correlation analysis; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2019.02.032
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Codonopsis Radix is a commonly used traditional Chinese medicine, and has the effect of strengthening spleen and tonifying lung, nourishing blood and engendering liquid. In addition, it is also used as important food materials. Aim of the study: The aim of the study was to explain the underlying correlations between chemical constituents and pharmacological effects and explore the bioactive markers of Codonopsis Radix. Materials and methods: Codonopsis Radix samples from Min county, Gansu province processed with different methods were taken as the materials, UPLC-ESI-Q-TOF-MS/MS analysis was conducted to identify the compounds and establish UPLC fingerprint. Meanwhile, hematopoietic and immunologic functions of Codonopsis Radix were investigated to obtain relevant pharmacological index. Then, the correlation analysis between chemical constituents in UPLC fingerprints and pharmacological effects was carried out. The plant name was confirmed to the database "The Plant List" (www.theplantlist.org ). Results: According to the results of canonical correlation analysis, tryptophan, syringin, tangshenoside I, codonopyrrolidium A, lobetyolin and two unknown compounds might be the potential bioactive markers related to the hematopoietic and immunologic functions of Codonopsis Radix, which could be recommended as the index compounds. Conclusion: This study illustrated the underlying correlations between chemical constituents and pharmacological effects, explored the pharmacological material basis, and could lay a foundation for the improvement of quality standard of Codonopsis Radix. Graphical abstract: fx1