Bax inhibitor-1 from orange spotted grouper, Epinephelus coioides involved in viral infection. Issue 78 (July 2018)

• Record Type: Journal Article
• Authors: Cai, Jia; Wei, Shina; Lu, Yishan; Wu, Zaohe; Qin, Qiwei; Jian, Jichang
• Is Part Of: Fish & shellfish immunology. Issue 78(2018)
• Page Start: 91
• Page End: 99
• Publication Date: 2018-07
• Subjects: Epinephelus coioides -- Bax inhibitor-1 (BI-1) -- Viral infection; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.04.020
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Bax inhibitor-1 (BI-1) is a conserved anti-apoptotic protein that suppresses endoplasmic reticulum (ER) stress-induced cell death. However, the function of fish BI-1 is not quite clear. In the present study, a bi-1 homolog (Ec bi-1 ) from orange spotted grouper, Epinephelus coioides was identified and its roles in viral infection were investigated. EcBI-1 encoded 237 amino acids protein, contained six transmembrane regions and a conservative C-terminus motif. Ec bi-1 predominantly expressed in kidney and spleen of healthy grouper. After SGIV stimulation, Ec bi-1 transcript was significantly increased in vitro . Subcellular localization analysis revealed that EcBI-1 was localized throughout the cytoplasm and co-localized with ER. Furthermore, overexpression of EcBI-1 suppressed SGIV infection induced cell death, caspase-3 activity and viral genes transcription. And C-terminus motif was critical for regulation roles of EcBI-1 during SGIV infection. In addition, EcBI-1 could interact with EcBNIP3 in vitro . Together, our data firstly demonstrated that fish BI-1 play important roles in response to viral infection. Highlights: Ec bi -1 was cloned and characterized. EcBI-1 was an ER-localized protein. EcBI-1 overexpression inhibited viral replication. EcBI-1 interacted with EcBNIP3 in vitro.