C21 steroidal glycosides from Cynanchum auriculatum and their neuroprotective effects against H2O2-induced damage in PC12 cells. (August 2017)

• Record Type: Journal Article
• Authors: Qian, Xicheng; Li, Baocai; Li, Peng; Wang, Dong; Dai, Weifeng; Zhang, Mi
• Is Part Of: Phytochemistry. Volume 140(2017)
• Page Start: 1
• Page End: 15
• Publication Date: 2017-08
• Subjects: Cynanchum auriculatum -- Apocynaceae -- C21 steroidal glycosides -- Neuroprotecton -- PC12 -- Apoptosis; ole oleandropyranose -- cym cymaropyranose -- digit digitoxopyranose; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2017.04.014
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Twenty-three undescribed previously C21 steroidal glycosides, named saccatols D–K and cynsaccatols I−W, together with six known analogs were isolated from the roots of Cynanchum auriculatum Royle ex Wight (Apocynaceae). Their structures were elucidated by a variety of spectroscopic techniques, as well as HRESIMS and GC method. All isolated compounds were tested their neuroprotective effects on the model of H2 O2 -induced damage to PC12 cells. Except saccatols D–F, J, cynsaccatol V and wilfoside A, other twenty-three isolates produced significantly activities against H2 O2 -induced cell damage (P < 0.001). Furthermore, cynsaccatols I, N, O and S can obvious inhibition of damaged PC12 cell apoptosis at their dosages of 1 μ M by Annexin V-FITC/PI double staining assay with flow cytometry. Graphical abstract: Image 1 Highlights: Twenty-three C21 steroids and six known analogs were isolated from Cynanchum auriculatum. Twenty-three C21 steroids produced significantly neuroprotective effects in vitro. Four steroids showed inhibition of injured PC12 apoptosis by Annexin V/PI double staining assay.