Complement component Bf/C2b gene mediates immune responses against Aeromonas hydrophila in grass carp Ctenopharyngodon idella. Issue 74 (March 2018)

• Record Type: Journal Article
• Authors: Dang, Yunfei; Shen, Yubang; Xu, Xiaoyan; Wang, Shentong; Meng, Xinzhan; Zhang, Meng; Lv, Liqun; Wang, Rongquan; Li, Jiale
• Is Part Of: Fish & shellfish immunology. Issue 74(2018)
• Page Start: 509
• Page End: 516
• Publication Date: 2018-03
• Subjects: Bf/C2b -- Grass carp -- Primary hepatic cell -- Aeromonas hydrophila -- Innate immunity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.01.030
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The complement system is a significant component of innate immunity. Here, we identified a Bf/C2 homolog ( gcBf/C2b ) in grass carp. gcBf/C2b shares a high similarity with Bf/C2b counterparts in other teleosts. gcBf/C2b transcription was widely distributed in different tissues, induced by Aeromonas hydrophila in vivo and in vitro, and affected by lipopolysaccharide and flagellin stimulation in vitro . In cells over-expressing gcBf/C2b, transcript levels of all components except gcC5 were significantly enhanced, and gcBf/C2b, gcIL1β, gcTNF-α, gcIFN, gcCD59, gcC5aR1, and gcITGβ-2 were significantly upregulated after A. hydrophila challenge or stimulation with bacterial pathogen-associated molecular patterns (PAMPs). However, gcBf/C2b in interference cells down-regulated the transcript levels after A. hydrophila challenge, and gcBf/C2b induced NF-κB signaling. These findings indicate the vital role of gcBf/C2b in innate immunity in grass carp. Highlights: We identified a C2 homolog ( gcC2 ) in grass carp. A. hydrophila induced gcC2 expression both in vivo and in vitro. Downregulation of gcC2 suppressed the immune response to A. hydrophila. Over-expression of gcC2 activated the NF-κB signaling pathway. Our findings indicate the vital role of gcC2 in innate immunity in grass carp.