White spot syndrome virus (WSSV) suppresses penaeidin expression in Marsupenaeus japonicus hemocytes. Issue 78 (July 2018)

• Record Type: Journal Article
• Authors: Zhang, Kehong; Koiwai, Keiichiro; Kondo, Hidehiro; Hirono, Ikuo
• Is Part Of: Fish & shellfish immunology. Issue 78(2018)
• Page Start: 233
• Page End: 237
• Publication Date: 2018-07
• Subjects: Marsupenaeus japonicus -- Hemocytes -- Penaeidin -- White spot syndrome virus (WSSV); Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.04.045
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Penaeidins are a unique family of antimicrobial peptides specific to penaeid shrimp and have been reported mainly function as anti-bacterial and anti-fungal. In order to investigate whether penaeidins could also respond to virus or not, we examined the effect of WSSV on MjPen-II (penaeidin in kuruma shrimp, Marsupenaeus japonicus ) expression. In the control group, MjPen-II transcript level can be detected in almost all test tissues but was expressed most strongly in hemocytes. After WSSV infection, MjPen-II transcript level was significantly downregulated in hemocytes. Moreover, the proportion of MjPen-II+ hemocytes was not significantly different between non-infected and WSSV-infected shrimp, but the number of MjPen-II+ highly expressing hemocytes decreased after infection. In addition, MjPen-II was observed in the cytoplasm of granule-containing hemocytes. These results suggest that WSSV suppresses MjPen-II expression in hemocytes. Highlights: MjPen-II transcript level can be detected in various tissues but was expressed most strongly in hemocytes. MjPen-II was located in the cytoplasm of granule-containing hemocytes in Marsupenaeus japonicus. MjPen-II transcript level was significantly downregulated after white spot syndrome virus (WSSV) infection. The proportion of MjPen-II+ highly expressing hemocytes decreased after WSSV infection but not with MjPen-II+ hemocytes.