Recombinant infectious hematopoietic necrosis virus expressing infectious pancreatic necrosis virus VP2 protein induces immunity against both pathogens. Issue 78 (July 2018)

• Record Type: Journal Article
• Authors: Guo, Mengting; Shi, Wen; Wang, Yanxue; Wang, Yuting; Chen, Yaping; Li, Dechuan; Ren, Xuanyu; Hua, Xiaojing; Tang, Lijie; Li, Yijing; Liu, Min
• Is Part Of: Fish & shellfish immunology. Issue 78(2018)
• Page Start: 187
• Page End: 194
• Publication Date: 2018-07
• Subjects: Reverse genetics -- Recombinant infectious hematopoietic necrosis virus (IHNV) -- Infectious pancreatic necrosis virus (IPNV) -- NV protein -- Immunogenicity; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2018.04.047
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Infectious hematopoietic necrosis virus (IHNV) and infectious pancreatic necrosis virus (IPNV) are typical pathogens of rainbow trout. Their co-infection is also common, which causes great economic loss in juvenile salmon species. Although vaccines against IHNV and IPNV have been commercialized in many countries, the prevalence of IHNV and IPNV is still widespread in modern aquaculture. In the present study, two IHNV recombinant viruses displaying IPNV VP2 protein (rIHNV-IPNV VP2 and rIHNV-IPNV VP2COE) were generated using the RNA polymerase Ⅱ system to explore the immunogenicity of IHNV and IPNV. The recombinant IHNV viruses were stable, which was confirmed by sequencing, indirect immunofluorescence assay, western blotting, transmission electron microscopy and viral growth curve assay. IHNV and IPNV challenge showed that the recombinant viruses had high protection rates against IHNV and IPNV with approximately 65% relative percent survival rates. Rainbow trout (mean weight 20 g) vaccinated with these two recombinant viruses showed a high level of antibodies against IHNV and IPNV infection. Taken together, our findings demonstrate that rIHNV-IPNV VP2 and rIHNV-IPNV VP2COE might be promising vaccine candidates against IHNV and IPNV. Highlights: Two IHNV recombinant viruses displaying IPNV VP2 protein were rescued. Two recombinant viruses had high protection rates against IHNV or IPNV. Two recombinant viruses both induce a high level of antibodies against IHNV and IPNV infection in vivo . Two recombinant viruses might be promising vaccine candidates against IHNV and IPNV.