Characterization of a Kazal-type serine protease inhibitor from black rockfish Sebastes schlegelii and its possible role in hepatic immune response. Issue 74 (March 2018)

• Record Type: Journal Article
• Authors: Kwon, Hyukjae; Yang, Hyerim; Lee, Seongdo; Nilojan, Jehanathan; Bathige, S.D.N.K.; Nam, Bo-Hye; Wan, Qiang; Lee, Jehee
• Is Part Of: Fish & shellfish immunology. Issue 74(2018)
• Page Start: 485
• Page End: 490
• Publication Date: 2018-03
• Subjects: Sebastes schlegelii -- Gene expression -- Kazal type serine protease inhibitor -- Serine protease; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2017.12.068
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Kazal-type serine protease inhibitors (KSPIs) play important roles in the regulation of endogenous proteases, cell development, blood coagulation, and immune response. In this study, we identified and characterized a KSPI homologue ( SsKSPI ) in black rockfish, Sebastes schlegelii . The full-length cDNA sequence of SsKSPI was 532 base pairs (bp), including an open reading frame (ORF) of 330 bp, which encodes a polypeptide of 110 amino acids with a signal peptide of 21 amino acids. The greatest value for identity (42.9%) and similarity (50.9%) was observed with Channa striata KSPI. We purified the recombinant protein of SsKSPI and performed protease inhibitory assays using three common serine proteases. The recombinant SsKSPI exhibited specific inhibitory activity against subtilisin A in a dose-dependent manner. Tissue distribution of SsKSPI mRNA has been examined amongst 10 important tissues in healthy rockfish and the liver was found to be the predominant expression organ of SsKSPI. The modulation of SsKSPI expression under immune challenges was also investigated in the liver. The SsKSPI mRNA expression was significantly up-regulated in response to both bacterial ( Streptococcus iniae and lipopolysaccharide) and viral (polyinosinic:polycytidylic acid) challenges. Overall, we propose that SsKSPI is potentially involved in the hepatic immune response against bacterial and viral infections in black rockfish. Highlights: A KSPI homologue ( SsKSPI ) was identified in Sebastes schlegelii. The recombinant SsKSPI specifically inhibited subtilisin A activity. The highest constitutive expression of SsKSPI was detected in the liver. SsKSPI gene expression was significantly inducible upon bacterial and viral challenges. SsKSPI is potentially involved in the hepatic immune response in black rockfish.