Enhanced crude oil depletion by constructed bacterial consortium comprising bioemulsifier producer and petroleum hydrocarbon degraders. (June 2019)

• Record Type: Journal Article
• Authors: Xia, Mingqian; Fu, Dafang; Chakraborty, Romy; Singh, Rajendra Prasad; Terry, Norman
• Is Part Of: Bioresource technology. Volume 282(2019)
• Page Start: 456
• Page End: 463
• Publication Date: 2019-06
• Subjects: Bioemulsifier -- Rhodococcus erythropolis -- Constructed bacterial consortium -- Crude oil depletion -- alkB gene; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.01.131
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Rhodococcus erythropolis OSDS1 produced highly stable bioemulsifier. Emulsification capacity of bioemulsifier was diesel > mineral/crude oil > gasoline. Mixed bacterial consortium containing OSDS1/TPH degraders was constructed. Consortium achieved 85.26% crude oil depletion efficiency in 15 days. Presence of alkane hydroxylase gene ( alkB ) was detected in the consortium. Abstract: The aim of this work was to study the production of bioemulsifier by Rhodococcus erythropolis OSDS1, and the improvement of crude oil depletion efficiency using a consortium of petroleum hydrocarbon degraders and OSDS1. The results showed that R. erythropolis OSDS1 produced highly stable bioemulsifier under various salinity (0–35 g/L NaCl) and pH (5.0–9.0) conditions; more than 90% of the initial emulsification activity was retained after 168 h. Emulsification capacity of the bioemulsifier on different petroleum hydrocarbons was diesel > mineral oil/crude oil > gasoline. A mixed bacterial consortium combining OSDS1 and four other petroleum hydrocarbon degraders was constructed. GC–MS results revealed that the constructed consortium achieved 85.26% depletion efficiency of crude oil in 15 days, which was significantly higher than that of individual strains. During the process, alkane hydroxylase gene ( alkB ) was successfully amplified from the consortium, confirming presence of crude oil degrading enzymes.