Transmission of mcr-1-Producing Multidrug-resistant Enterobacteriaceae in Public Transportation in Guangzhou, China. (13th November 2018)

• Record Type: Journal Article
• Authors: Shen, Cong; Feng, Siyuan; Chen, Hongtao; Dai, Min; Paterson, David L; Zheng, Xiaobin; Wu, Xingui; Zhong, Lan-Lan; Liu, Yan; Xia, Yong; Ma, Rui; Huang, Xi; Tian, Guo-Bao
• Is Part Of: Clinical infectious diseases. Volume 67(2018)Supplement 2
• Page Start: S217
• Page End: S224
• Publication Date: 2018-11-13
• Subjects: mcr-1 -- Escherichia coli -- Klebsiella pneumonia -- public transportation; Communicable diseases -- Periodicals; 616.905
• DOI: 10.1093/cid/ciy661
• Languages: English
• ISSNs: 1058-4838

Abstract

Abstract: Objectives: mcr-1 -mediated colistin resistance in bacteria is concerning, as colistin is used in treating multidrug-resistant bacterial infections. And mcr-1 -producing bacteria have been identified in multiple sources. Up to 248 million people use public transportation daily in China, however; public transportation hasn't been studied as a potential source of community-based transmission of mcr-1 . Herein we investigated mcr-1 -producing isolates from public transportation and explored the genomic characteristics of them. Methods: Surface samples were collected from public transportation in Guangzhou, China, from October 2016 to April 2017. Polymerase chain reaction was performed to detect mcr-1 gene, plasmid replicon type and phylogenetic group. Minimum inhibitory concentrations (MICs) were determined by microdilution method. S1-nuclease digestion and pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting were performed with mcr-1 -harboring plasmids. Whole-genome sequencing was performed with mcr-1 -producing isolates. Results: Of the 737 samples with bacterial growth, 26 isolates were positive for mcr-1, including 23 Escherichia coli and 3 Klebsiella pneumoniae isolates. The E. coli isolates belonged to phylogroups A and B1. Most mcr-1 -producing isolates were resistant to ampicillin (25), cefotaxime (21), fosfomycin (16), and gentamicin (15). S1-PFGE, Southern blotting and replicon typing showed that mcr-1 was mainly located on ~33.3 kb to ~220 kb IncX4, IncI2 and IncHI2 plasmids in E. coli, while located on ~33.3 kb untyped plasmid in K. pneumoniae . Several sequence types (ST), including ST2253, ST101, ST10 complex and ST37, were revealed. Between 53 and 66 (mean = 61.8) resistance genes were identified among mcr-1 -producing isolates. Conclusions: Public transportation may serve as a source of mcr-1 -producing bacteria.