Compatibility of Maximum-Containment Virus–Inactivation Protocols With Identification of Bacterial Coinfections by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. (4th July 2018)

• Record Type: Journal Article
• Authors: Matson, M Jeremiah; Stock, Frida; Shupert, W Lesley; Bushmaker, Trenton; Feldmann, Friederike; Bishop, Wendy B; Frank, Karen M; Dekker, John P; Chertow, Daniel S; Munster, Vincent J
• Is Part Of: Journal of infectious diseases. Volume 218(2018)Supplement 5
• Page Start: S297
• Page End: S300
• Publication Date: 2018-07-04
• Subjects: Ebola -- maximum containment virus -- inactivation -- MALDI-TOF MS -- bacterial co-infection -- sepsis; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiy292
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Diagnostics and research analyses involving samples containing maximum-containment viruses present unique challenges, and inactivation protocols compatible with downstream testing are needed. Our aim was to identify a validated viral inactivation protocol compatible with bacterial identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We assessed a panel of bacteria with 6 validated maximum-containment virus–inactivation protocols and report that inactivation with TRIzol or γ-irradiation is compatible with MALDI-TOF MS. The availability, simplicity, and rapidity of TRIzol inactivation make this method the more suitable choice.