PSIII-36 Dietary soybean meal level and β-mannanase supplementation affected immunoproteins in carotid artery and morphology and aquaporin water channels in small intestine of nursery pigs. (7th December 2018)

• Record Type: Journal Article
• Authors: Qiao, Y; Zhu, X; Zhai, L; Payne, R; Li, T
• Is Part Of: Journal of animal science. Volume 96(2018)Supplement 3
• Page Start: 313
• Page End: 314
• Publication Date: 2018-12-07
• Subjects: SBM -- β-mannanase -- pig; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/sky404.690
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: One hundred Landrace × Yorkshire barrows (BW Mean 9.49 kg, SD 1.18 kg) were used in a thirty-day 2 × 2 RCBD trial to study the effects of soybean meal (SBM) and β-mannanase (Hemicell XT) on small intestine. Treatments were SBM (220 and 370 g/kg, making dietary soluble β-mannan to 2420 and 4070 ppm, respectively) and β-mannanase (0 and 200 mg/kg). Diets were isocaloric (3200 kcal/kg ME) and equal in SID Lys (12.5 g/kg). On Day 30, six pigs per treatment were sacrificed for blood from mesenteric vein, portal vein and carotid artery to measure serum IgM, IgG and complement C4 (C4) and for small intestine to measure morphology and relative mRNA of aquaporins (AQP1, AQP3, AQP8, AQP10, AQP11) that channel transmembrane water movement. Data were analyzed with GLM of SPSS. In carotid artery, β-mannanase increased C4 (P<0.01) and SBM tended to increase IgM (p=0.08). Treatment had no other effects on the measured immuno-proteins in the specified serum. In jejunum, SBM decreased villus height (P=0.02) whereas β-mannanase increased it (P<0.01). Treatment did not affect jejunal crypt depth. SBM tended to decrease jejunal villus/crypt (V/C) (P=0.07) whereas β-mannanase increased it (P=0.03). In ileum, SBM decreased villus height (P=0.02) whereas β-mannanase increased it (P=0.01). SBM and β-mannanase had no main effects on either crypt depth or V/C in the ileum but SBM × β-mannanase affected them (P=0.01). В-mannanase restored ileal crypt depth and V/C to the same levels as low SBM diets in pigs fed high SBM feed. In jejunum, SBM decreased AQP11 (P=0.01) and β-mannanase increased AQP8 (p<0.01) and AQP10 (P=0.02). In ileum, SBM decreased AQP3, AQP8 and AQP11 (P<0.01) whereas β-mannanase increased AQP3 (P=0.01) and AQP11 (P=0.02) and tended to increase AQP8 (P=0.06). Dietary SBM and β-mannanase appeared to be counteractive on small intestinal morphology and function in the pigs.