PSXV-9 A Novel Anti-sense LncRNA of CEBPA Inhibits Bovine Adipogenic Differentiation. (7th December 2018)

• Record Type: Journal Article
• Authors: Li, A; Hu, Y; Liu, X; Zhao, L; Tian, Q; Du, M
• Is Part Of: Journal of animal science. Volume 96(2018)Supplement 3
• Page Start: 245
• Page End: 245
• Publication Date: 2018-12-07
• Subjects: cattle -- lncRNA -- adipogenesis; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/sky404.535
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: Long non-coding RNAs (lncRNAs) have been revealed to play key role in the adipogenesis. Recently, a number of lncRNAs have been identified in adipose tissue by RNA sequencing, however the function of these lncRNAs remains largely undefined. In this study, we identified a novel bovine lncRNA located on bovine chromosome 18. It spans approximately 4.25 kb on the genome, containing two exons and one intron. The transcript is about 2608 bp which is an anti-sense lncRNA of CCAAT/enhancer-binding protein α gene (CEBPA), the product of which is known to regulate adipogenesis. To investigate the potential role of this LncRNA in adipogenesis, we cloned the anti-sense lncRNA of CEBPA into the pcDNA3.1+ vector. Then the construct was transfected into the bovine stromal vascular fraction (SVF) cells. Six hours (h) after transfection, an adipogenic inducing medium was applied. After 48 h following transfection, cells were collected. Compared to the pcDNA3.1+ vector only transfection, anti-sense lncRNA of CEBPA was dramatically enhanced due to LncRNA plasmid transfection (P < 0.01), while CEBPA and PPARγ2 were significantly down-regulated (P < 0.05). Oil Red O staining showed the over-expression of anti-sense lncRNA of CEBPA inhibited lipid accumulation in differentiated SVF cells. Interestingly, when we cloned the anti-sense lncRNA of CEBPA into the nuclear pZW1-snoVector, which restricts expressed lncRNA to the nuclei, the same inhibitory effect was observed, suggesting that this lncRNA targets genomic DNA. In conclusion, the anti-sense lncRNA of CEBPA inhibits adipogenesis in the bovine SVF cells.