302 Targeted sequencing approach identifies immune loci associated to resistance to Haemonchus contortus in sheep and goats. (7th December 2018)

• Record Type: Journal Article
• Authors: Estrada Reyes, Z; Tsukahara, Y; Goetsch, A; Gipson, T; Wang, Z; Sahlu, T; Puchala, R; Leal Gutierrez, J; Mateescu, R
• Is Part Of: Journal of animal science. Volume 96(2018)Supplement 3
• Page Start: 114
• Page End: 115
• Publication Date: 2018-12-07
• Subjects: Genetics -- sheep -- goats; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/sky404.251
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: The aim of this study was to identify single nucleotide polymorphisms (SNPs) associated to internal parasite resistance in sheep and goats exposed to H. contortus, using a targeted sequencing approach. Three different breeds of sheep (St. Croix, Katahdin and Dorper) and goats (Spanish, Boer and Kiko) were used in this study. Dewormed male animals were infected with 10, 000 L3 H. contortus larvae. Average daily gain (ADG), fecal egg count (FEC) and package cell volume (PCV) were measured in all animals. The level of IgA, IgM and IgG was measured from serum samples at 21 days post-infection. Targeted sequencing panel included 100 candidate genes for immune response against H. contortus . SNPs were discarded if call rate < 95% and minor allele frequency ≤0.01. A mixed model was used to analyze the response variables and included the identity by state matrix. Year and breed were included as fixed effects. Bonferroni correction was used to control for multiple testing. Thirty two SNPs on chromosomes 1, 2, 5, 11, 12, 16, 17, 19, 24 and X were significant for different traits. For ADG and IgM, significant SNPs were located within IL12RB2 and IL4R genes. For IgA and PCV, one common peak was significant to both traits in exon 2 of CD86 gene. For IgG and IgM, significant regions were observed on IL5RA. Significant regions for FEC and PCV were identified in the NFIL3 gene. These results suggest that chromosomal regions related to cytokine receptors and membrane proteins expressed by antigen presenting cells, play an important role in the expression of resistant phenotypes. In conclusion, potential immune like loci could be used as DNA markers for resistance in sheep and goats exposed to H. contortus .