The effect of surface charge and pH on the physiological behaviour of cobalt, copper, manganese, antimony, zinc and titanium oxide nanoparticles in vitro. (August 2018)

• Record Type: Journal Article
• Authors: Titma, Tiina
• Is Part Of: Toxicology in vitro. Volume 50(2018)
• Page Start: 11
• Page End: 21
• Publication Date: 2018-08
• Subjects: ATP adenosine triphosphate -- BET Brunauer–Emmett–Teller method -- BSA bovine serum albumin -- CCM complete culture medium -- DLS dynamic light scattering -- ELS electrophoretic light scattering -- FBS fetal bovine serum -- IEP isoelectric point -- LDH lactate dehydrogenase -- MeOx metal oxide -- MilliQ MilliQ water (Millipore, 18 MΩ) -- MTS 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium -- NEAA non-essential amino acids -- PEST penicillin streptocide -- NMTR non-monotonic time response -- NP nanoparticle -- SDS sodium dodecyl sulphate; Surface charge -- Hydrodynamic size -- Caco2 model cells -- Long term nanotoxicity -- Nonmonotonic time response (NMTR); Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2018.02.010
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: The precise knowledge on various interactions of metal nanoparticles (NP) in a living organism is scarce. It is expected that metals can bind to nucleic acids, peptides and proteins ( e.g. enzymes), and modify the functioning of vital cellular compartments after entering the organism. The predictive factors for quantitative nanostructure-activity relationship (QNAR) analysis could enhance efficient and harmless usage of nanoparticles (NPs) in the industry as well in the medicine. The studies value the composition of the NP corona determined by time, temperature and source of protein which has been found to implicate the physiological behaviour of NPs. One has largely been ignored: the NPs specific isoelectric point (IEP) and pH at the state of measurement. Herein, this study investigates the effect of pH and surface charge of six metal oxide (MeOx) NPs on time dependency of cytotoxicity. Several aspects of the characterization of ultrafine particles in the actual test system which is the most relevant for the interpretation of the toxicological data are referred: (i) the difference of pH in the room temperature and in the incubation conditions (ii) the difference of dispersions in MilliQ and complete cell media; (iii) the need to exemplify also the pH and isoelectric point when the hydrodynamic size is measured; (iv) the importance of time due to the time-dependent equilibration and changes of NPs corona. The surface charge determines the formation of corona and could be modified by pH. MeOx NPs without fully charge equilibrated corona might play the main role of MeOx NPs entering into the cell and consequently the time dependent manifestation of the cellular effect. Highlights: The behaviour of metal oxide nanoparticles could be modified by surface charge and pH. The toxicity is relevant to be evaluated using the actual conditions of the physiological environment. The remarkable toxicity of xenobiotics might emerge after the essential 24 h as shown here in the case of antimony.