Enhancing oleanolic acid production in engineered Saccharomyces cerevisiae. (June 2018)

• Record Type: Journal Article
• Authors: Zhao, Yujia; Fan, Jingjing; Wang, Chen; Feng, Xudong; Li, Chun
• Is Part Of: Bioresource technology. Volume 257(2018)
• Page Start: 339
• Page End: 343
• Publication Date: 2018-06
• Subjects: Oleanolic acid -- Oxidation-reduction system -- Galactose metabolic network; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.02.096
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Reconstructing oleanolic acid biosynthesis pathway in Saccharomyces cerevisiae. Boosting oleanolic acid production by pairing different CPRs with MtCYP716A12. Boosting oleanolic acid production by reconstructing galactose metabolic network. Oleanolic acid titer was 606.9 ± 9.1 mg/L, 7.6-fold higher than previous report. Provide a platform strain for biosynthesis of oleanane type triterpenoids. Abstract: Oleanolic acid is a plant-derived pentacyclic triterpenoid compound with various biological activities. Recently, biosynthesis of oleanolic acid in microbes has been demonstrated as a promising and green way, but the production is too low for industrialization. To improve oleanolic acid production, this study constructed a novel pathway for biosynthesis of oleanolic acid in Saccharomyces cerevisiae by improving the pairing efficiency between cytochrome P450 monooxygenase and reductase. Furthermore, to improve the transcriptional efficiency of heterologous genes, the cellular galactose regulatory network was reconstructed by knocking out galactose metabolic genes GAL80 and GAL1 . Finally, the 3-hydroxy-3-methylglutaryl-CoA reductase, squalene synthase and 2, 3-oxidosqualene synthase were further overexpressed, increasing oleanolic acid production up to 186.1 ± 12.4 mg/L in flask shake. Combined with fermentation optimization, the final oleanolic acid production was 606.9 ± 9.1 mg/L with a yield of 16.0 ± 0.8 mg/g DCW which was 7.6-fold higher than the reported maximum production.