Mixotrophic and heterotrophic production of lipids and carbohydrates by a locally isolated microalga using wastewater as a growth medium. (June 2018)

• Record Type: Journal Article
• Authors: Nzayisenga, Jean Claude; Eriksson, Karolina; Sellstedt, Anita
• Is Part Of: Bioresource technology. Volume 257(2018)
• Page Start: 260
• Page End: 265
• Publication Date: 2018-06
• Subjects: Biodiesel -- Chlorella -- FTIR -- Heterotrophy -- Wastewater; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2018.02.085
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Mixotrophic condition with glycerol showed the highest biomass. Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) can be used to analyze algal FTIR data. The highest lipid content was observed in heterotrophic condition using glycerol. The highest carbohydrate content was obtained in autotrophic condition. Biodiesel quality increases in heterotrophic condition using glycerol. Abstract: The biomass production and changes in biochemical composition of a locally isolated microalga ( Chlorella sp.) were investigated in autotrophic, mixotrophic and heterotrophic conditions, using glucose or glycerol as carbon sources and municipal wastewater as the growth medium. Both standard methods and Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) analysis of data acquired by Fourier-transform IR (FTIR) spectrometry showed that autotrophic and mixotrophic conditions promoted carbohydrate accumulation, while heterotrophic conditions with glycerol resulted in the highest lipid content and lowest carbohydrate content. Heterotrophic conditions with glycerol as a carbon source also resulted in high oleic acid (18:1) contents and low linolenic acid (18:3) contents, and thus increasing biodiesel quality. The results also show the utility of MCR-ALS for analyzing changes in microalgal biochemical composition.