Canavalia gladiata and Arctium lappa extracts ameliorate dextran sulphate sodium-induced inflammatory bowel disease by enhancing immune responses. (June 2018)

• Record Type: Journal Article
• Authors: Ji, Kon-Young; Jang, Ji-Hye; Lee, Eun-Hee; Kim, Su-Man; Song, Hyeong-Woo; Yang, Won-Kyung; Kim, Han-Young; Kim, Kun-Hoae; Lee, Young-Sil; Kim, Dong-Seon; Kang, Hyung-Sik; Kim, Seung-Hyung
• Is Part Of: Journal of functional foods. Volume 45(2018)
• Page Start: 24
• Page End: 33
• Publication Date: 2018-06
• Subjects: Canavalia gladiate -- Arctium lappa -- Dextran sulphate sodium-induced inflammatory bowel disease -- Immune response; DSS dextran sulphate sodium -- IBD inflammatory bowel disease -- ROS reactive oxygen species -- NO nitric oxide -- LPS lipopolysaccharide -- NK natural killer -- CD Crohn's disease -- UC ulcerative colitis -- ATCC America Type Culture Collection -- FBS foetal bovine serum -- PBS phosphate-buffered saline, DCF-DA, 2′, 7′-dichlorofluorescin diacetate -- RT room temperature -- CMC carboxymethylcellulose -- WT wild type -- FACS fluorescence-activated cell sorting -- TMB tetramethylbenzidine -- OCT optimal cutting temperature -- PDA photodiode array detector -- LDH lactate dehydrogenase; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2018.03.018
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: C. gladiata and A. lappa, and their mixture had an anti-inflammation activity. C. gladiata and A. lappa and their mixture enhanced immune response in WT. Mixture of C. gladiate and A. lappa, can provide benefit in DSS-induced IBD models. The marker compounds can restore functional defect in DSS-induced IBD models. Abstract: Canavalia gladiata ( C. gladiata ) and Arctium lappa ( A. lappa ), used in traditional medicine and food. However, their preventive effects in dextran sulphate sodium (DSS)-induced inflammatory bowel disease (IBD) remain unclear. We found that the C. gladiata and A. lappa mixture suppressed inflammation in LPS-induced RAW 264.7 cells. The mixture increased the population and activation of immune cells, up-regulation of cell cycle, and induction of IgA and IgG production in wild-type mice. Furthermore, the extract mixture prevented clinical IBD symptoms and restored IgA production in IBD mice. Similarly, a mixture of the marker compounds, chicoric acid and lupeol, ameliorated IBD-associated clinical signs, improved the population and activation of immune cells and functional defects in natural killer (NK) cells and reduced production of IgA in these mice. In conclusion, the C. gladiata and A. lappa mixture ameliorated progression of DSS-induced IBD by enhancing immune responses and recovering functional immune cell defects.