0093 Dynamics Of Cortical Sleep Spindles And Slow Waves Following Motor Learning In Mice. (27th April 2018)

• Record Type: Journal Article
• Authors: Kam, K; Pettibone, W; Varga, A
• Is Part Of: Sleep. Volume 41(2018)Supplement 1
• Page Start: A37
• Page End: A38
• Publication Date: 2018-04-27
• Subjects: Sleep -- Physiological aspects -- Periodicals; Sleep disorders -- Periodicals; Sommeil -- Aspect physiologique -- Périodiques; Sommeil, Troubles du -- Périodiques; Sleep disorders; Sleep -- Physiological aspects; Sleep -- physiological aspects; Sleep Wake Disorders; Psychophysiology; Electronic journals; Periodicals; 616.8498
• DOI: 10.1093/sleep/zsy061.092
• Languages: English
• ISSNs: 0161-8105

Abstract

Abstract: Introduction: Increased cortical sleep spindles and slow waves during NREM sleep have been observed in offline improvement during human motor learning. We investigated sleep spindles and slow waves in offline gains in motor learning in mice. Methods: C57/Bl6 mice (7 male, 4 female) performed 10 trials/day on an accelerating rotarod at ZT 0 on 2 consecutive days. Offline changes were calculated by comparing the average of the last 3 trials on day 1 to the first 3 trials of day 2. Between days 1 and 2, mice were recorded with video-polysomnography with EEG leads targeted over bilateral primary motor cortex under conditions of ad libitum sleep. Results: Sleep following rotarod learning was marked by an increase in spindle count (237 ± 18 following learning vs 272 ± 23 following handling, n = 11, p = 0.023) from 1 to 4 hours after NREM sleep onset, without significant change in spindle peak frequency, duration, coherence, or relative power in the spindle band (10–16 Hz). There was a significantly greater number of spindles in the first 1 to 4 hours of NREM sleep in female mice (304 ± 23 spindles, n = 4) compared to male mice (212 ± 10 spindles, n = 7, t-test: p < 0.001) following rotarod learning. Cross-correlational coupling between peak spindle power and delta power was not found to be different during NREM sleep after motor learning and control sleep. The degree of rotarod offline performance change was associated with the number of spindles in early NREM sleep in male mice (r=0.802, p=0.030) however this association was weaker and no longer significant when taking into account both sexes (r=0.573, p=0.071). Conclusion: Ad libitum sleep following rotarod motor learning results in an early increase in spindle count and density compared to sleep without motor learning. The association between offline performance change and early spindle count may be sex-specific. Support (If Any): ISMMS.