PSX-33 Effects of cellulase-producing microorganisms isolated from silkworm (Bombyx mori) on growth performance and gut microbial community composition of weaned piglets. (7th December 2018)

• Record Type: Journal Article
• Authors: Guevarra, R; Lee, S; Marimuthu, V; Lee, J; Kim, S; Baek, J; Mun, D; Kim, J; Paek, S; Jung, S; Song, M; Kim, H
• Is Part Of: Journal of animal science. Volume 96(2018)Supplement 3
• Page Start: 488
• Page End: 488
• Publication Date: 2018-12-07
• Subjects: Pig -- silkworm -- growth performance -- microbiota; Livestock -- Periodicals; Livestock; Electronic journals; Periodicals; 636.005
• DOI: 10.1093/jas/sky404.1066
• Languages: English
• ISSNs: 0021-8812

Abstract

Abstract: This study was conducted to evaluate the effects of cellulase-producing microorganisms isolated from silkworm ( Bombyx mori ) on growth performance and gut microbial community composition of weaned piglets. A total of 12 weaned pigs (7.17 ± 0.63 kg BW; 28 d old) were randomly assigned to 2 dietary treatments (1 pig/pen; 6 pens/treatment) in a complete randomized design. The dietary treatments were 1) a control diet based on corn and soybean meal (CON) and 2) CON + cellulase-producing bacteria ( L. lactis 10 8 CFU/mL). The screening for cellulase-producing bacteria in silkworm gut was done by carboxymethylcellulose (CMC) plate method. The dietary treatments did not include spray dried plasma, fishmeal, zinc oxide, and antibiotics to avoid their antibacterial or physiological effects. Pigs were fed respective dietary treatments for 6 weeks. Fecal samples were collected from randomly selected 1 pig per replicate on d 1, 7, 14, 21, and 42 after weaning. Data for growth performance were analyzed using the PROC GLM procedure of SAS. The statistical model for growth performance included dietary effect as a fixed effect. The total DNA extracted from fresh fecal samples was used to amplify the 16S rRNA gene for sequencing in Illumina MiSeq. Sequencing data was quality filtered and analyzed using QIIME software. Cellulase-producing bacteria enhanced growth performance during overall experimental period compared with CON. The fecal microbiome of the weaned piglets treated with cellulase-producing bacteria showed higher relative abundance of bacteria in the genus Prevotella and Lactobacillus compared to control pigs. In conclusion, cellulase-producing bacteria did not significantly improved growth rate of weaned pigs, however, it altered the gut microbial community of weaned piglets.