Experimental design for single-cell RNA sequencing. (8th November 2017)

• Record Type: Journal Article
• Authors: Baran-Gale, Jeanette; Chandra, Tamir; Kirschner, Kristina
• Is Part Of: Briefings in functional genomics. Volume 17:Number 4(2018:Jul.)
• Page Start: 233
• Page End: 239
• Publication Date: 2017-11-08
• Subjects: single-cell RNA sequencing -- Smart-Seq 2 -- 10x Chromium -- Drop-Seq -- experimental design; Genomics -- Methodology -- Periodicals; Genomics -- Technological innovations -- Periodicals; 572.86072
• DOI: 10.1093/bfgp/elx035
• Languages: English
• ISSNs: 2041-2649

Abstract

Abstract: Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.