A Sensitive LC–MS-MS Method for Quantification of 1, 6-O, O-Diacetylbritannilactone in Rat Plasma and its Application in a Pharmacokinetic Study. (29th December 2017)

• Record Type: Journal Article
• Authors: Zhou, Qiang; Yan, He; Li, Rui; Li, Xingliang; Wei, Jingyan
• Is Part Of: Journal of chromatographic science. Volume 56:Number 3(2018:Mar.)
• Page Start: 242
• Page End: 247
• Publication Date: 2017-12-29
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmx106
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: 1, 6- O, O -Diacetylbritannilactone is a natural sesquiterpene lactone isolated from Inula britannica that has displayed cytotoxic effects against several human cancer cell lines. In this study, a selective and sensitive liquid chromatography–tandem mass spectrometry (LC–MS-MS) method was developed for determination of 1, 6- O, O -diacetylbritannilactone in rat plasma. Chromatographic separation was achieved on an Agilent C18 column (4.6 mm × 75 mm, 3.5 μm) with methanol and water (80:20, v / v ) as the mobile phase. An ESI source was applied and operated in positive ion mode; selected-reaction monitoring was used for quantification using target fragment ions m / z 373.2→312.9 for 1, 6- O, O -diacetylbritannilactone and m / z 331.2→144.1 for the IS. Calibration plots were linear in the range of 1.5–1350 ng/mL for 1, 6- O, O -diacetylbritannilactone in rat plasma. Intra- and inter-day precisions were <8.5%, and the accuracy ranged from −2.7 to 12.8%. The LC–MS-MS method was successfully applied in a pharmacokinetic study of 1, 6- O, O -diacetylbritannilactone in rats.