Binding of Fibronectin to SsPepO Facilitates the Development of Streptococcus suis Meningitis. (15th December 2017)

• Record Type: Journal Article
• Authors: Liu, Feng; Li, Jinquan; Yan, Kang; Li, Huan; Sun, Chengfeng; Zhang, Shuo; Yuan, Fangyan; Wang, Xiangru; Tan, Chen; Chen, Huanchun; Bei, Weicheng
• Is Part Of: Journal of infectious diseases. Volume 217:Number 6(2018)
• Page Start: 973
• Page End: 982
• Publication Date: 2017-12-15
• Subjects: fibronectin -- meningitis -- RGD peptide -- SsPepO -- Streptococcus suis; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jix523
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract : In this study, we showed that the SsPepO-fibronectin-integrin interaction is important for S suis adherence to human brain microvascular endothelial cells, which facilitates the development of S suis meningitis. Abstract: Background: SsPepO is an important virulence in Streptococcus suis . Methods: In this study, we showed that SsPepO contributes to the human fibronectin-mediated adherence ability of S. suis to human brain microvascular endothelial cells. Results: The addition of an antifibronectin antibody or an arginine-glycine-aspartic acid peptide that blocks fibronectin binding to integrins significantly reduced adherence of the wild-type but not the SspepO mutant strain, indicating the importance of the SsPepO-fibronectin-integrin interaction for S. suis cellular adherence. Conclusions: By analyzing Evans blue extravasation in vivo, we showed that the interaction between SsPepO and human fibronectin significantly increased permeability of the blood-brain barrier. Furthermore, the SspepO mutant caused lower bacterial loads in the brain than wild-type S. suis in models of meningitis. These data demonstrate that SsPepO is a fibronectin-binding protein, which plays a contributing role in the development of S. suis meningitis.