Knockdown of timeless Disrupts the Circadian Behavioral Rhythms in Laodelphax striatellus (Hemiptera: Delphacidae). Issue 5 (27th July 2018)
- Record Type:
- Journal Article
- Title:
- Knockdown of timeless Disrupts the Circadian Behavioral Rhythms in Laodelphax striatellus (Hemiptera: Delphacidae). Issue 5 (27th July 2018)
- Main Title:
- Knockdown of timeless Disrupts the Circadian Behavioral Rhythms in Laodelphax striatellus (Hemiptera: Delphacidae)
- Authors:
- Jiang, Yan-Dong
Yuan, Xin
Bai, Yue-Liang
Wang, Gui-Yao
Zhou, Wen-Wu
Zhu, Zeng-Rong - Abstract:
- Abstract: Most living organisms developed the innate clock system to anticipate daily environmental changes and to enhance their chances of survival. timeless ( tim ) is a canonical clock gene. It has been extensively studied in Drosophila melanogaster (Diptera: Drosophilidae) as a key component of the endogenous circadian clock, but its role is largely unknown in some agriculture pests. Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae), an important rice pest, exhibits a robust locomotor rhythm. In the present study, we cloned tim gene ( ls-tim ) from L. striatellus and investigated its function in the regulation of behavioral rhythms. Quantitative real-time polymerase chain reaction revealed a circadian expression pattern of ls-tim under different light conditions with a trough in the photophase and a peak in the late scotophase. After the knockdown of ls-tim via RNA interference (RNAi), the adults showed an earlier onset of locomotor activity under light/dark cycles and became arrhythmic in constant darkness. ls-tim RNAi also abolished the timing of adult emergence that normally occurs in the early photophase. These results suggest that ls-tim is essential for the light-entrained circadian rhythms in L. striatellus and provide more insights into the endogenous clock network underlying the behavioral and physiological rhythms of this insect.
- Is Part Of:
- Environmental entomology. Volume 47:Issue 5(2018)
- Journal:
- Environmental entomology
- Issue:
- Volume 47:Issue 5(2018)
- Issue Display:
- Volume 47, Issue 5 (2018)
- Year:
- 2018
- Volume:
- 47
- Issue:
- 5
- Issue Sort Value:
- 2018-0047-0005-0000
- Page Start:
- 1216
- Page End:
- 1225
- Publication Date:
- 2018-07-27
- Subjects:
- circadian clock -- timeless -- locomotor activity -- adult emergence -- RNA interference
Beneficial insects -- Periodicals
Beneficial insects -- United States -- Periodicals
Insect pests -- Control -- Periodicals
Entomology -- Periodicals
632.7 - Journal URLs:
- http://ee.oxfordjournals.org/content/ ↗
http://www.oxfordjournals.org ↗ - DOI:
- 10.1093/ee/nvy095 ↗
- Languages:
- English
- ISSNs:
- 0046-225X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3791.464000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 12205.xml