A re-sequencing-based ultra-dense genetic map reveals a gummy stem blight resistance-associated gene in Cucumis melo. (7th September 2017)

• Record Type: Journal Article
• Authors: Hu, Zhongyuan; Deng, Guancong; Mou, Haipeng; Xu, Yuhui; Chen, Li; Yang, Jinghua; Zhang, Mingfang
• Is Part Of: DNA research. Volume 25:Number 1(2018:Feb.)
• Page Start: 1
• Page End: 10
• Publication Date: 2017-09-07
• Subjects: ultra-dense genetic map -- re-sequencing -- gummy stem blight -- Cucumis melo; Genes -- Periodicals; Genomes -- Periodicals; Genes -- Research -- Periodicals; Genomics -- Periodicals; DNA -- Research -- Periodicals; 572.86072
• DOI: 10.1093/dnares/dsx033
• Languages: English
• ISSNs: 1340-2838

Abstract

Abstract: The melon ( Cucumis melo ) genome and genetic maps with hundreds to thousands of single nucleotide polymorphism markers were recently released. However, a high-resolution genetic map was lacking. Gummy stem blight (Gsb) is a destructive disease responsible for considerable economic losses during melon production. We herein describe the development of an ultra-dense genetic map consisting of 12, 932 recombination bin markers covering 1, 818 cM, with an average distance of 0.17 cM between adjacent tags. A comparison of the genetic maps for melon, watermelon, and cucumber revealed chromosome-level syntenic relationships and recombination events among the three Cucurbitaceae species. Our genetic map was useful for re-anchoring the genome scaffolds of melon. More than 92% assembly was anchored to 12 pseudo-chromosomes and 90% of them were oriented. Furthermore, 1, 135 recombination hotspots revealed an unbalanced recombination rate across the melon genome. Genetic analyses of the Gsb-resistant and -susceptible lines indicated the resistance phenotype is mediated by a single dominant gene. We identified Gsb-resistance gene candidates in a 108-kb region on pseudo-chromosome 4. Our findings verify the utility of an ultra-dense genetic map for mapping a gene of interest, and for identifying new disease resistant genes.