Comparative genomics of Czech vaccine strains of Bordetella pertussis. Issue 7 (3rd September 2018)

• Record Type: Journal Article
• Authors: Dienstbier, Ana; Pouchnik, Derek; Wildung, Mark; Amman, Fabian; Hofacker, Ivo L; Parkhill, Julian; Holubova, Jana; Sebo, Peter; Vecerek, Branislav
• Is Part Of: Pathogens and disease. Volume 76:Issue 7(2018)
• Publication Date: 2018-09-03
• Subjects: Bordetella -- pertussis -- genomics -- region of difference -- genome rearrangement -- vaccine pressure; Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1093/femspd/fty071
• Languages: English
• ISSNs: 2049-632X

Abstract

Abstract: Bordetella pertussis is a strictly human pathogen causing the respiratory infectious disease called whooping cough or pertussis. B. pertussis adaptation to acellular pertussis vaccine pressure has been repeatedly highlighted, but recent data indicate that adaptation of circulating strains started already in the era of the whole cell pertussis vaccine (wP) use. We sequenced the genomes of five B. pertussis wP vaccine strains isolated in the former Czechoslovakia in the pre-wP (1954–1957) and early wP (1958–1965) eras, when only limited population travel into and out of the country was possible. Four isolates exhibit a similar genome organization and form a distinct phylogenetic cluster with a geographic signature. The fifth strain is rather distinct, both in genome organization and SNP-based phylogeny. Surprisingly, despite isolation of this strain before 1966, its closest sequenced relative appears to be a recent isolate from the US. On the genome content level, the five vaccine strains contained both new and already described regions of difference. One of the new regions contains duplicated genes potentially associated with transport across the membrane. The prevalence of this region in recent isolates indicates that its spread might be associated with selective advantage leading to increased strain fitness. Abstract : This is the first report on the genomics of Czech pertussis vaccine strains showing their uniqueness in terms of SNP-based phylogeny and genome organization.