Methanol induces cytosolic calcium variations, membrane depolarization and ethylene production in arabidopsis and tobacco. (20th March 2018)

• Record Type: Journal Article
• Authors: Tran, Daniel; Dauphin, Aurélien; Meimoun, Patrice; Kadono, Takashi; Nguyen, Hieu T H; Arbelet-Bonnin, Delphine; Zhao, Tingting; Errakhi, Rafik; Lehner, Arnaud; Kawano, Tomonori; Bouteau, François
• Is Part Of: Annals of botany. Volume 122:Number 5(2018)
• Page Start: 849
• Page End: 860
• Publication Date: 2018-03-20
• Subjects: Arabidopsis thaliana -- calcium -- methanol -- signalization -- volatile; Botany -- Periodicals; 580
• DOI: 10.1093/aob/mcy038
• Languages: English
• ISSNs: 0305-7364

Abstract

Abstract: Background and Aims: Methanol is a volatile organic compound released from plants through the action of pectin methylesterases (PMEs), which demethylesterify cell wall pectins. Plant PMEs play a role in developmental processes but also in responses to herbivory and infection by fungal or bacterial pathogens. However, molecular mechanisms that explain how methanol could affect plant defences remain poorly understood. Methods: Using cultured cells and seedlings from Arabidopsis thaliana and tobacco BY2 expressing the apoaequorin gene, allowing quantification of cytosolic Ca 2+, a reactive oxygen species (ROS) probe (CLA, Cypridina luciferin analogue) and electrophysiological techniques, we followed early plant cell responses to exogenously supplied methanol applied as a liquid or as volatile. Key Results: Methanol induces cytosolic Ca 2+ variations that involve Ca 2+ influx through the plasma membrane and Ca 2+ release from internal stores. Our data further suggest that these Ca 2+ variations could interact with different ROS and support a signalling pathway leading to well known plant responses to pathogens such as plasma membrane depolarization through anion channel regulation and ethylene synthesis. Conclusions: Methanol is not only a by-product of PME activities, and our data suggest that [Ca 2+ ]cyt variations could participate in signalling processes induced by methanol upstream of plant defence responses.