Increased cytokines production and oxidative stress are related with purinergic signaling and cell survival in post-thyroidectomy hypothyroidism. (1st January 2020)

• Record Type: Journal Article
• Authors: Baldissarelli, Jucimara; Mânica, Aline; Pillat, Micheli Mainardi; Bagatini, Margarete Dulce; Leal, Daniela Bitencourt Rosa; Abdalla, Fátima Husein; Morsch, Vera M.; Ulrich, Henning; Bornemann, Clarissa Pereira; Chitolina Schetinger, Maria R.
• Is Part Of: Molecular and cellular endocrinology. Volume 499(2019)
• Publication Date: 2020-01-01
• Subjects: Hypothyroidism -- Cytokines -- Reactive species -- Ectonucleotidases -- Inflammatory processes; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2019.110594
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Thyroid hormones have essential roles in regulation of cellular functions, including the immune system. The purinergic signaling, activated through extracellular nucleotides and nucleosides has also strong implications in immune response regulation. Hypothyroidism may involve effects on the immune and purinergic systems. In view of that, we evaluated cytokines levels, their relation with the expression of purinergic enzymes and the effects of this condition on immune system cells from patients with post-thyroidectomy hypothyroidism. Increased IL6, IL10, IL17 and TNF-α levels as well as an increase in CD73 expression in lymphocytes were observed in patients' blood. Moreover, augmented myeloperoxidase activity, lipid peroxidation and thiolgroup production were observed in post-thyroidectomy hypothyroidism. In addition, proliferation and cell death of lymphocytes were enhanced when exposed to patients' serum. This study demonstrates that hypothyroidism is related to changes in the purinergic system, increased cytokines production and oxidative stress, which interfere in the cell life and signaling. Highlights: Post-thyroidectomy hypothyroidism increases CD73 expression, changing purinergic signaling. High cytokines levels and oxidative stress causes an inflammatory status in hypothyroidism. Hypothyroidism increases the susceptibility and death of cells, improving antioxidant defenses.