High in vivo levels of adipsin lead to increased knee tissue degradation in osteoarthritis: data from humans and animal models. (29th June 2018)

• Record Type: Journal Article
• Authors: Valverde-Franco, Gladys; Tardif, Ginette; Mineau, François; Paré, Frédéric; Lussier, Bertrand; Fahmi, Hassan; Pelletier, Jean-Pierre; Martel-Pelletier, Johanne
• Is Part Of: Rheumatology. Volume 57:Number 10(2018)
• Page Start: 1851
• Page End: 1860
• Publication Date: 2018-06-29
• Subjects: adipsin -- osteoarthritis -- alternative complement pathway -- cartilage -- synovial membrane; Rheumatism -- Periodicals; Rheumatology -- Periodicals; 616.723005
• DOI: 10.1093/rheumatology/key181
• Languages: English
• ISSNs: 1462-0324

Abstract

Abstract: Objective: This study explored the role of the adipokine adipsin in OA. Methods: Control and OA articular tissues, cells and serum were obtained from human individuals. Serum adipsin levels of human OA individuals were compared with cartilage volume loss as assessed by MRI at 48 months. Human adipsin expression was determined by PCR, its production in tissues by immunohistochemistry, and in SF and serum by a specific assay. OA was surgically induced in wild-type ( Df +/+ ) and adipsin-deficient (Df −/− ) mice, and synovial membrane and cartilage processed for histology and immunohistochemistry. Results: Adipsin levels were significantly increased in human OA serum, SF, synovial membrane and cartilage compared with controls, but the expression was similar in chondrocytes, synoviocytes and osteoblasts. Multivariate analysis demonstrated that human serum adipsin levels were significantly associated ( P = 0.045) with cartilage volume loss in the lateral compartment of the knee. Destabilization of the medial meniscus- Df −/− mice showed a preservation of the OA synovial membrane and cartilage lesions ( P ⩽ 0.026), the latter corroborated by the decreased production of cartilage degradation products and proteases ( P ⩽ 0.047). The adipsin effect is likely due to a deficient alternative complement pathway ( P ⩽ 0.036). Conclusion: In human OA, higher serum adipsin levels were associated with greater cartilage volume loss in the lateral compartment, and adipsin deficiency led to a preservation of knee structure. Importantly, we documented an association between adipsin and OA synovial membrane and cartilage degeneration through the activation of the complement pathway. This study highlights the clinical relevance of adipsin as a valuable biomarker and potential therapeutic target for OA.