High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. (28th June 2019)

• Record Type: Journal Article
• Authors: Mollerup, Sarah; Asplund, Maria; Friis-Nielsen, Jens; Kjartansdóttir, Kristín Rós; Fridholm, Helena; Hansen, Thomas Arn; Herrera, José Alejandro Romero; Barnes, Christopher James; Jensen, Randi Holm; Richter, Stine Raith; Nielsen, Ida Broman; Pietroni, Carlotta; Alquezar-Planas, David E; Rey-Iglesia, Alba; Olsen, Pernille V S; Rajpert-De Meyts, Ewa; Groth-Pedersen, Line; von Buchwald, Christian; Jensen, David H; Gniadecki, Robert; Høgdall, Estrid; Langhoff, Jill Levin; Pete, Imre; Vereczkey, Ildikó; Baranyai, Zsolt; Dybkaer, Karen; Johnsen, Hans Erik; Steiniche, Torben; Hokland, Peter; Rosenberg, Jacob; Baandrup, Ulrik; Sicheritz-Pontén, Thomas; Willerslev, Eske; Brunak, Søren; Lund, Ole; Mourier, Tobias; Vinner, Lasse; Izarzugaza, Jose M G; Nielsen, Lars Peter; Hansen, Anders Johannes;
• Is Part Of: Journal of infectious diseases. Volume 220:Number 8(2019)
• Page Start: 1312
• Page End: 1324
• Publication Date: 2019-06-28
• Subjects: cancer -- enrichment -- human -- virome; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiz318
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: Background: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data. Methods: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads. Results: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found. Conclusions: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference. Abstract : High-throughput sequencing of approximately 200 cancer samples detected viruses from 7 viral families. More than half of the investigated samples contained 1 or more viruses; however, no associations linking specific viruses with specific cancer types were found.