A FRET-based two-photon probe for in vivo tracking of pH during a traumatic brain injury process. (23rd October 2019)

• Record Type: Journal Article
• Authors: Zhai, Baoping; Zhai, Shuyang; Hao, Ruilin; Xu, Jianjun; Liu, Zhihong
• Is Part Of: New journal of chemistry. Volume 43:Number 43(2019)
• Page Start: 17018
• Page End: 17022
• Publication Date: 2019-10-23
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c9nj04049h
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Traumatic brain injury (TBI) is a cause of neurodegenerative diseases accompanied by intracellular pH decrease. Herein, a FRET-based ratiometric two-photon fluorescent pH probe is designed to monitor pH change and understand TBI process. Abstract : The traumatic brain injury (TBI) process, a significant cause of neurodegenerative diseases, is generally thought to be accompanied by a decrease in pH of the intracellular environment. In this work, a fluorescence resonance energy transfer (FRET)-based ratiometric two-photon fluorescent pH probe, FRET-pH, has been designed and fully characterized to monitor the pH change so as to understand the extent of the TBI process. FRET-pH, which has nearly 100% energy transfer efficiency and ∼100-fold ratio signal changes, is constructed from a naphthalene derivative ADN as a two-photon fluorophore and SRhB as a recognition domain for pH. FRET-pH displays both one- and two-photon ratiometric responses towards pH in aqueous solution. The appropriate p K a value (5.89) supports its use in subsequent biological applications. Finally, two-photon microscopic imaging of pH in living HeLa cells, BV-2 cells and rat brain tissue has been achieved.