Tracking mitochondrial viscosity in living systems based on a two-photon and near red probe. (21st October 2019)

• Record Type: Journal Article
• Authors: Peng, Min; Yin, Junling; Lin, Weiying
• Is Part Of: New journal of chemistry. Volume 43:Number 43(2019)
• Page Start: 16945
• Page End: 16949
• Publication Date: 2019-10-21
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c9nj03744f
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A two-photon fluorescence probe, Mito-V, with near-red emission to measure the viscosity changes of mitochondria in living cells and in vivo . Abstract : A two-photon fluorescent probe, Mito-V, was designed and prepared for sensing the viscosity changes of mitochondria. The systematic investigations demonstrated that the fluorescence emission at 634 nm increased about 22-fold from methanol to 99% glycerol, indicating high viscosity dependence. Notably, using the probe, the mitochondrial viscosity fluctuations have been successfully monitored not only in living cells but also in zebrafish and living mice.