Performance Evaluation and Clinical Associations of Immunoassays That Detect Antibodies to Negatively Charged Phospholipids Other Than Cardiolipin. Issue 5 (14th March 2018)

• Record Type: Journal Article
• Authors: Castanon, Amaris; Pierre, Grant; Willis, Rohan; Harris, E Nigel; Papalardo, Elizabeth; Romay-Penabad, Zurina; Schleh, Alvaro; Jajoria, Praveen; Smikle, Monica; DeCeulaer, Karel; Tebo, Anne; Jaskowski, Troy; Guerra, Marta M; Branch, D Ware; Salmon, Jane E; Petri, Michelle; Gonzalez, Emilio B
• Is Part Of: American journal of clinical pathology. Volume 149:Issue 5(2018)
• Page Start: 401
• Page End: 411
• Publication Date: 2018-03-14
• Subjects: Antiphospholipid -- Anticardiolipin -- APhL -- Antiphosphatidylserine -- Antiphosphatidylinositol -- Assay -- Noncriteria; Diagnosis, Laboratory -- Periodicals; Pathology -- Periodicals; 616.07
• DOI: 10.1093/ajcp/aqy003
• Languages: English
• ISSNs: 0002-9173

Abstract

Abstract: Objectives: We evaluate the performance characteristics of antiphosphatidylserine (anti-PS), antiphosphatidylinositol (anti-PI), and antiphospholipid mixture (APhL) enzyme-linked immunosorbent assays (ELISAs) compared with anticardiolipin (aCL) and anti–β2 glycoprotein I (anti-β2GPI) in a large group of patients with antiphospholipid (aPL)–related diseases. Methods: Serum samples from 548 patients from the Hopkins and Jamaican systemic lupus erythematosus cohorts, the PROMISSE cohort, and the Antiphospholipid Standardization Laboratory were examined for immunoglobulin G (IgG)/immunoglobulin M (IgM) positivity in aCL, anti-β2GPI, anti-PS, anti-PI, and APhL ELISA assays. Results: All IgG assays were associated with one or more thrombotic and/or obstetric manifestations, with an increased risk associated with higher antibody titers. Analytical performance was similar among assays, but IgG assays performed better than IgM counterparts. Conclusions: Increasing titers of APhL, anti-PS, and anti-PI antibodies could indicate an increased risk of thrombotic and/or obstetric aPL-related manifestations. These assays may be promising biomarkers for particular APS manifestations.