Aptamer/magnetic nanoparticles decorated with fluorescent gold nanoclusters for selective detection and collection of human promyelocytic leukemia (HL-60) cells from a mixture. (15th October 2019)

• Record Type: Journal Article
• Authors: Haghighi, Farid Hajareh; Binaymotlagh, Roya; Mirahmadi-Zare, Seyede Zohreh; Hadadzadeh, Hassan
• Is Part Of: Nanotechnology. Volume 31:Number 2(2020)
• Publication Date: 2019-10-15
• Subjects: magnetic nanoparticles -- dual fluorescent/MRI imaging -- cell separation -- KH1C12 aptamer -- HL-60 cell line -- poly(ethylene glycol) dimethacrylate; Nanotechnology -- Periodicals; Nanotechnology -- Periodicals; Nanotechnology; Publications périodiques; Nanotechnologies; Periodicals; 620.5
• DOI: 10.1088/1361-6528/ab484a
• Languages: English
• ISSNs: 0957-4484

Abstract

Abstract: In the present work, a fluorescent gold nanoclusters (GNCs)/superparamagnetic (Fe3 O4 /GNCs) nanoprobe was prepared via a facile approach for the selective detection and imaging of human leukemica cancer cells (HL-60). ( γ -Mercaptopropyl)trimethoxysilane (MPS) was used as a stabilizer to prepare functionalized GNCs. The prepared GNCs@MPS was then self-assembly decorated on the surface of Fe3 O4 @SiO2 nanoparticles followed by poly(ethylene glycol) dimethacrylate (PGD) addition at room temperature to form Fe3 O4 /GNCs nanoprobe. Surface functionalization of the Fe3 O4 /GNCs with the thiol-modified KH1C12 aptamer was done through thiol-en click reaction between PGD and the thiol group of the aptamer. An extensive characterization of the Fe3 O4 /GNCs revealed strong red fluorescence ( λ em = 627 nm), T 2 -based contrast agent for MRI and excellent colloidal and photo stability in buffer medium. So, the aptamer-functionalized Fe3 O4 /GNCs nanoprobe (Fe3 O4 /GNCs/Aptamer) is capable to uptake and dual-image HL-60 cancer cells from a mixture. Furthermore, the MRI signal intensity of the pictures decreased linearly with an increase in the concentrations of the nanoprobe. It is also enable to detect cancer cells from a range of concentrations 10 up to 200 cells μ L −1 . The fluorescent/magnetic characteristics of the nanoprobe are of great significance for MRI-based and fluorescence imaging and collection of HL-60 cancer cells which implies potential help for the development of early diagnosis of highly malignant human leukemia.