Expression of a novel versican variant in dorsal root ganglia from spared nerve injury rats. (September 2019)

• Record Type: Journal Article
• Authors: Bogen, Oliver; Bender, Olaf; Alvarez, Pedro; Kern, Marie; Tomiuk, Stefan; Hucho, Ferdinand; Levine, Jon D
• Is Part Of: Molecular pain. Volume 15(2019)
• Publication Date: 2019-09
• Subjects: Versican -- nerve injury -- neuropathic pain -- nonsense mediated RNA decay -- eIF2α(PO4)2- -- Isolectin B4; Pain -- Molecular aspects -- Periodicals; Pain -- Pathophysiology -- Periodicals; Pain -- Physiological aspects -- Periodicals; 616.0472
• DOI: 10.1177/1744806919874557
• Languages: English
• ISSNs: 1744-8069

Abstract

The size and modular structure of versican and its gene suggest the existence of multiple splice variants. We have identified, cloned, and sequenced a previously unknown exon located within the noncoding gene sequence downstream of exon 8. This exon, which we have named exon 8β, specifies two stop-codons. mRNAs of the versican gene with exon 8β are predicted to be constitutively degraded by nonsense-mediated RNA decay. Here, we tested the hypothesis that these transcripts become expressed in a model of neuropathic pain.