In vitro fermentation of Cucumis sativus fructus extract by canine gut microbiota in combination with two probiotic strains. (December 2019)

• Record Type: Journal Article
• Authors: Belà, Benedetta; Coman, Maria Magdalena; Verdenelli, Maria Cristina; Bianchi, Cinzia; Pignataro, Giulia; Fiorini, Dennis; Silvi, Stefania
• Is Part Of: Journal of functional foods. Volume 63(2019)
• Publication Date: 2019-12
• Subjects: Cucumis sativus -- Probiotic strains -- Prebiotics -- Canine gut microbiota -- Fermentation; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103585
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Cucumis sativus fructus extract modify dog gut microbiota in vitro fermentation. Probiotics act in synergy with C. sativus f.e. on dog gut microbiota fermentation. Fibre and probiotics increase Bifidobacterium and Lactobacillus spp. counts in vitro. SCFAs production and ammonia increased during supplement in vitro fermentation. Studied supplements have some prebiotic-like effects on dog gut microbiota. Abstract: There is a growing public interest in the use of fibre, probiotics and combinations of both of them to potentially improve the gut microbiota composition and the digestive health of dogs; however, there are limited studies on the effects of these supplements in companion animals. Using 24 h in vitro fermentation, the effects of probiotic strains, Lactobacillus paracasei JCM8130 and Lactobacillus acidophilus C8.1, and the fructus extract of Cucumis sativus on modulation and activities of healthy dogs' microbiota were investigated, either individually or in combination, to evaluate their possible synergism. Real-Time PCR was performed for enumeration of the selected bacterial groups. Gas chromatographic analysis was conducted to quantify short chain fatty acids (SCFAs) production. The studied supplements showed some prebiotic-like effects especially the ability to promote a significant increase of Bifidobacterium spp. and Lactobacillus spp. counts and also the production of acetic, propionic and butyric acids.