A Coumarin-Based Fluorescence Probe for Selective Recognition of Cu2+ Ions and Live Cell Imaging. (30th October 2019)

• Record Type: Journal Article
• Authors: He, Guangjie; Ma, Nana; Li, Linlin; Xie, Chenyan; Yang, Linlin; Xu, Jinhe; Wang, Junli
• Other Names: Ji Hai-Feng Academic Editor.
• Is Part Of: Journal of sensors. Volume 2019(2019)
• Publication Date: 2019-10-30
• Subjects: Detectors -- Periodicals; 681.205
• DOI: 10.1155/2019/2814947
• Languages: English
• ISSNs: 1687-725X

Abstract

Abstract : A new fluorescence probe L was rationally designed and synthesized for the recognition of Cu 2+ ions by the combination of coumarin hydrazide and 2-acetylpyrazine. The photochemical properties and selectivity of L for Cu 2+ ions in a CH3 CN/HEPES (3 : 2, v / v ) buffer were investigated by UV-vis absorption and fluorescence emission spectra. A highly selective and sensitive response of L for Cu 2+ ions over other competing metal ions was observed with limit of detection in 3 μ M. The coordination stoichiometry of L to Cu 2+ ions was determined to be 1 : 1 by the UV-vis absorption spectrum, the fluorescence titrations, and density functional theory (DFT) calculations. Moreover, L was applied successfully for recognition of intracellular Cu 2+ ions in living cells.