Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model. (3rd November 2019)

• Record Type: Journal Article
• Authors: Li Calzi, Sergio; Cook, Todd; Della Rocca, Domenico G.; Zhang, Juan; Shenoy, Vinayak; Yan, Yuanqing; Espejo, Andrew; Rathinasabapathy, Anandharajan; Jacobsen, Max H.; Salazar, Tatiana; Sandusky, George E.; Shaw, Lynn C.; March, Keith; Raizada, Mohan K.; Pepine, Carl J.; Katovich, Michael J.; Grant, Maria B.
• Other Names: Deutsch Marcus-André Academic Editor.
• Is Part Of: Stem cells international. Volume 2019(2019)
• Publication Date: 2019-11-03
• Subjects: Stem Cells -- Periodicals; Stem Cells -- Therapeutic use -- Periodicals; Stem Cells -- Transplantation -- Periodicals; 616.0277405
• DOI: 10.1155/2019/3945850
• Languages: English
• ISSNs: 1687-966X

Abstract

Abstract : We compared the functional outcome of Isl-1 + cardiac progenitors, CD90 + bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1 + cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1 + /CD90 + cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1 + cells, CD90 + cells, or a combination of Isl-1 + and CD90 + cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1 + cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90 + cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1 + or CD90 + cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1 + cardiac progenitors and adult bone marrow-derived CD90 + cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair.