A folic acid modified polystyrene nanosphere surface for circulating tumor cell capture. Issue 44 (29th October 2019)

• Record Type: Journal Article
• Authors: Chen, Changchong; Wang, Zhili; Zhao, Yuewu; Cao, Yi; Ding, Pi; Liu, Hui; Su, Na; Pei, Renjun
• Is Part Of: Analytical methods. Volume 11:Issue 44(2019)
• Page Start: 5718
• Page End: 5723
• Publication Date: 2019-10-29
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c9ay02090j
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A polystyrene nanosphere (PSNP) substrate was developed to capture CTCs from blood samples, and the captured target cells were proliferated using an in situ culture strategy. Abstract : Circulating tumor cells (CTCs) have been considered as a significant biomarker for cancer metastasis and relapse. Effective detection of CTCs will play an important role in early cancer diagnosis, prognosis, progress monitoring, and personalized therapy. In this work, we developed a polystyrene (PS) nanosphere substrate to capture CTCs from whole blood samples. Bovine serum albumin (BSA), as an antifouling molecule, and folic acid, as a recognizing agent, were modified onto the surface of PS nanospheres via self-polymerization of dopamine. 8 HeLa cells could be captured when 10 cells were spiked into 1 mL of the whole blood sample. Then, the captured target cells were further proliferated through an in situ culture strategy on the polystyrene (PS) nanosphere substrate, and nearly 80 HeLa cells were obtained from 1 captured cell after 7 days of in situ culture, revealing the potential application for CTC isolation and proliferation.