Dissecting human gliomas by single-cell RNA sequencing. Issue 1 (7th July 2017)

• Record Type: Journal Article
• Authors: Tirosh, Itay; Suvà, Mario L
• Is Part Of: Neuro-oncology. Volume 20:Issue 1(2018)
• Page Start: 37
• Page End: 43
• Publication Date: 2017-07-07
• Subjects: diffuse gliomas -- heterogeneity -- single-cell RNA sequencing; Brain Neoplasms -- Periodicals; Brain -- Tumors -- Periodicals; Brain -- Cancer -- Periodicals; Nervous system -- Cancer -- Periodicals; 616.99481
• DOI: 10.1093/neuonc/nox126
• Languages: English
• ISSNs: 1522-8517

Abstract

Abstract: Diffuse gliomas are the most common human primary brain tumors and remain incurable. They are complex entities in which diverse genetic and nongenetic effects determine tumor biology and clinical course. Our current understanding of gliomas in patients is primarily based on genomic and transcriptomic methods that have profiled them as bulk, providing critical information yet masking the diversity of cells within each tumor. Recent advances in single-cell DNA and RNA profiling have paved the way to studying tumors at cellular resolution. Here, we review initial studies deploying single-cell analysis in clinical glioma samples, with a focus on RNA expression profiling. We highlight how these studies provide new insights into glioma biology, tumor heterogeneity, cancer cell lineages, cancer stem cell programs, the tumor microenvironment, and glioma classification.